development and application of a validated liquid chromatography-mass spectrometry method for the determination of dexchlorpheniramine maleate in human plasma

a convenient liquid chromatographic-single quadrupole mass spectrometric (lc-ms) method was developed and validated for dexchlorpheniramine maleate (inn name: chlorphenamine) determination in human plasma. the need for just a single liquid-liquid extraction with ethyl acetate and being highly sensitive were the advantages of this method. the linearity was also excellent over the range of 1 to 150 ng.ml-1 of dexchlorpheniramine maleate concentration. the method was statistically validated for its selectivity, linearity, precision and robustness. this method was successfully applied to the analysis of chlorpheniramine maleate in clinical studies.